Fig. 5

Scatterplots of the mean beta values (a, c, e, g) and volcano plots of each pairwise comparison (b, d, f, h) related to differential methylation among matched primary and metastatic samples, at the promoter level. a, b) patient #1; c, d) patient #2; e, f patient #3; g, h patient #4. In the scatterplots, the transparency corresponds to point density. Blue points represent differentially methylated sites (according to the combined rank criteria, see “Methods”). Red dots represent the 1000 best ranking sites. In the volcano plots, dots are colored according to combined rank, and the yy axis represents the combined p values (or the mean quotient log₂ when comparing two samples). Met metastatic samples, Prim primary tumor samples