Fig. 6

MRN complex affects DDR response to FK228 and radiation. a Relative expression of NBN in radioresistant SCLC cell lines compared to radiosensitive cell lines. b Relative expression of MRE11 expression at protein level in radioresistant SCLC cell lines compared to radiosensitive cell lines. c Western blot analysis of MRE11 showing that radioresistant SCLC cell lines have more MRE11 expression than radiosensitive cells. d Western blot analysis of MRE11, 53BP1, P-DNA-PKcs, P-RPA2 in SCLC Cells treated with radiation (4 Gy) (b), 1 nM FK228, or in combination. β-actin was used as a loading control. e Relative amount of proteins in SCLC Cells treated with radiation (4 Gy), 1 nM FK228, or in combination were determined by densitometric analysis. The quantification data presented were the average densitometric value of three independent western blotting experiments