Fig.1

FK228 induces apoptosis and cytotoxicity in a panel of SCLC cell lines. a Western blot analysis of acetylated H3 in SCLC cell lines after FK228 for 24 h. b Growth inhibition curves of FK228 in a panel of SCLC cell lines. CellTiter-Glo Luminescent assay was performed to evaluate the cell viability after treatment with FK228 for 72 h. The IC50 values were determined from the sigmoidal dose–response curves using PRISM5 software. c Annexin V apoptotic assay indicates that FK228 induces apoptosis. SCLC cells were treated with DMSO control, 0.1 nM, 1 nM, and 5 nM FK228 for 48 h. After treatment, Annexin V apoptotic assay was performed by flow cytometry. d Western blot analysis of cleaved PARP in three SCLC cell lines after FK228 for 24 h