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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Inhibition of HDAC4 by GSK3β leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration

Fig. 3

HDAC4 elevates ASK1 expression and thus promotes IDD. a ASK1 silencing efficiency determined by RT-qPCR; *p < 0.05 versus sh-NC. b Changes in disc height index (DHI). c Apoptosis reflected by TUNEL-positive cells. d HDAC4, ASK1, Bcl-2, Bax, and cleaved caspase-3 protein expression determined by Western blotting. e Mechanical pain threshold. f Thermal sensitivity threshold; *p < 0.05 versus control; #p < 0.05 versus oe-NC + sh-NC; &p < 0.05 versus oe-HDAC4 + sh-NC. n = 9 for animal experiments; cell experiments were performed in triplicate

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Clinical Epigenetics

ISSN: 1868-7083

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