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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Inhibition of HDAC4 by GSK3β leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration

Fig. 2

HDAC4 promotes ASK1 expression through KLF5. a KLF5 and ASK1 mRNA expression. *p < 0.05 versus control. b KLF5 and ASK1 protein expression determined by Western blotting; *p < 0.05 versus control. c Binding between HDAC4 and the KLF5 promoter determined by ChIP; KLF5-ChIP is the promoter chip. *p < 0.05 versus oe-NC. d KLF5 acetylation determined by IP. e Binding of gene promoter determined by ChIP; ASK1-ChIP is the promoter chip. *p < 0.05 versus oe-NC. f ASK1 mRNA expression determined by RT-qPCR. g ASK1 protein expression determined by Western blotting; *p < 0.05 versus sh-NC. h ASK1 protein expression determined by Western blotting following oe-HDAC4, sh-KLF5 or both. *Indicates p < 0.05 and **indicates p < 0.01. n = 9 for animal experiments; cell experiments were performed in triplicate

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Clinical Epigenetics

ISSN: 1868-7083

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