Fig. 7

Effect of HDAC or IL-6 inhibition on genes associated with immunosuppression and myeloid cell recruitment in CRC tumor tissues. Cells isolated from CRC tissue were cultured in the presence of LPS and HDAC inhibitor (100 nM) or anti-IL-6 mAb (2 μg/ml). The relative mRNA expression levels for HDAC1, HDAC2, HDAC3, HDAC4 (a), and ARG1, CCR2, IDO, ITGAL, Wnt5a, and Wnt5b were determined in the absence or presence of HDAC inhibitor (b). The relative mRNA expression levels for ARG1, CCR2, IDO, ITGAL, Wnt5a, and Wnt5b were determined in the absence or presence of anti-IL-6 mAb (c). The relative gene expression was normalized to β-actin. Results obtained from four CRC patients, #26, #56, #60 and #63, and expressed as mean ± SEM.