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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Transcriptomic profiling disclosed the role of DNA methylation and histone modifications in tumor-infiltrating myeloid-derived suppressor cell subsets in colorectal cancer

Fig. 2

Comparison of myeloid populations (PMN-MDSCs, I-MDSCs, M-MDSCs, and APCs) in NT and TT of CRC patients. Cells isolated from NT and TT of 27 CRC patients were stained for myeloid cell markers and analyzed by flow cytometry. Scatter plots show the relative percentages of CD33+HLA-DR−/lowCD14−CD15+ PMN-MDSCs, CD33+HLA-DR−/lowCD14−CD15− I-MDSCs, CD33+HLA-DR−/lowCD14+CD15− M-MDSCs, and CD33+HLA-DR+CD14+ APCs in NT and TT from 27 CRC patients (a). Scatter plots show differences in absolute numbers of PMN-MDSCs, I-MDSCs, M-MDSCs, and APCs in NT and TT from 27 CRC patients (b). Scatter plots show differences in relative percentages and absolute numbers of PMN-MDSCs, I-MDSCs, and M-MDSCs in TT from 27 CRC patients (c). Flow cytometric data were merged to create single t-distributed stochastic neighbor embedding (tSNE) maps to show PMN-MDSCs (denoted as PMN), I-MDSCs (denoted as I), M-MDSCs (denoted as M), and APCs in NT and TT (d). Representative flow cytometric plots show the gating strategy employed to define and sort PMN-MDSCs, I-MDSCs, M-MDSCs, and APCs from two CRC patients #07 (e) and #08 (f)

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Clinical Epigenetics

ISSN: 1868-7083

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