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Table 1 Distribution of low, intermediate and highly methylated DNA methylation sites between different genomic elements in NEC and non-NEC ileum tissues

From: Global hypermethylation of intestinal epithelial cells is a hallmark feature of neonatal surgical necrotizing enterocolitis

Feature Methylation Ileum
Percent NEC (%)a Non-NEC (%)a Chi-square2b df p
Promoter  < 20 37,570 (21.3) 37,809 (21.4) 5.1 2 0.079
20–80 67,544 (38.2) 67,963 (38.5)
 > 80 71,628 (40.5) 70,970 (40.2)
Exonic < 20 11,252 (4.7) 11,276 (4.7) 83.9 2 < 0.0001
20–80 105,227 (43.6) 108,315 (44.9)
> 80 124,986 (51.8) 121,874 (50.5)
Intronic < 20 83,095 (1.9) 84,504 (1.9) 2270 2 < 0.0001
20–80 1,880,459 (42.3) 1,949,492 (43.8)
> 80 2,483,522 (55.8) 2,413,080 (54.3)
CpG Island < 20 14,248 (45.5) 14,297 (45.6) 0.32 2 0.85
20–80 6478 (20.7) 6496 (20.7)
> 80 10,611 (33.9) 10,544 (33.6)
CpG Shore < 20 55,456 (15.9) 55,885 (16.0) 9.49 2 0.0087
20–80 125,323 (35.9) 124,102 (35.6)
> 80 168,110 (48.2) 168,902 (48.4)
Enhancer < 20 895 (7.6) 910 (7.7) 2.55 2 0.28
20–80 5807 (49.4) 5913 (50.3)
> 80 5047 (43.0) 4926 (41.9)
Intergenic < 20 80,112 (1.7) 82,289 (1.7) 7668 2 < 0.0001
20–80 2,462,179 (51.2) 2,595,021 (54.0)
> 80 2,264,520 (47.1) 2,129,501 (44.3)
CpG Island + CpG Shore < 20 69,704 (18.3) 70,182 (18.5) 8.62 2 0.014
20–80 131,801 (34.7) 130,598 (34.3)
> 80 178,721 (47.0) 179,446 (47.2)
Exons + Introns < 20 94,347 (2.0) 95,780 (2.0) 2349 2 < 0.0001
20–80 1,985,686 (42.4) 2,057,807 (43.9)
> 80 2,608,508 (55.6) 2,534,954 (54.1)
All < 20 205,779 (2.1) 208,548 (2.2) 12,306 2 < 0.0001
20–80 4,378,720 (45.3) 4,618,095 (47.8)
> 80 5,074,456 (52.5) 4,832,312 (50.0)
  1. df degrees of freedom, p probability
  2. aCount (percent)
  3. bPearson's Chi-squared test