Fig. 1

Deregulation of miR expression levels in a murine model of CMML. a To screen for aberrant miR expression profiles in CMML, we performed miR-microarray analysis in CD-11b⁻/Ly-6G⁻/c-Kit⁺ HSPCs isolated from the bone marrow of mice with a Ras-induced CMML-like MPD. Therefore, four Mx1-Cre⁺/Kras^G12D mice and four Kras^Wt control mice were injected with pIpC as outlined in the materials and methods section. Mice were killed and analyzed 6 weeks later. The development of the CMML-like MPD at this time was verified as outlined in Additional file 1: Fig S13. b Volcano plot showing deregulated miRs in CMML-HSPCs of Mx1-Cre⁺/Kras^G12D-mutated mice. The horizontal axis depicts the x-fold expression change in Mx1-Cre⁺/Kras^G12D-mutated animals and transformed P-values are depicted on the vertical axis. miRs with higher expression in Kras^G12D-mutated HSPCs are displayed on the right, whereas miRs with decreased expression levels in Kras^G12D-mutated HSPCs are displayed on the left. c–d qPCR validation of the miR microarray results shows decreased miR-125a, miR-150, and miR-26a expression in CD-11b⁻/Ly-6G⁻/c-Kit⁺ (c, n = 3) and Lin⁻/Sca-1⁺/c-Kit⁺ (d, n = 4) HSPC compartments of Mx1-Cre⁺/Kras^G12D mice. At least three mice per group were analyzed. The dot plots show the relative miR expression with the median indicated as horizontal line. One Kras^Wt mouse was always used as a calibrator and its expression is set to 1. Group differences were assessed by t test. Wt, wildtype;