Fig. 2
STXBP5-AS1 inhibited chemoresistance and metastasis of PC cells. a The overexpression efficiency of STXBP5-AS1 in Gemcitabine-resistant PANC-1 and Mia PaCa-2 (PANC-1/GR and Mia PaCa-2/GR) cells transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) was confirmed by qRT-PCR. b Determination of the effects of STXBP5-AS1 overexpression on Gemcitabine IC50 in PANC-1/GR and Mia PaCa-2/GR cells using MTT assay. c Quantification of colony formation in Gemcitabine-challenged (50 ng/ml) PANC-1/GR and Mia PaCa-2/GR cells transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector). d, e PANC-1/GR and Mia PaCa-2/GR cells transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) cells were treated with 50 ng/ml Gemcitabine for 48 h, the apoptosis was analyzed by caspase-3 activity assay and FACS. f The overexpression efficiency of STXBP5-AS1 in PANC-1 and Mia PaCa-2 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) was confirmed by qRT-PCR. g Transwell assay indicated that STXBP5-AS1 repressed the invasion of PANC-1 and Mia PaCa-2 cells. h and i H&E staining of the metastatic nodules in the lung of PANC-1 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) following tail vein injection into nude mice (200× scale bars) and incidence of lung metastasis in mice following tail vein injection of the respective PANC-1 cells. *P < 0.05; **P < 0.01 (χ2 test for I, Student’s t test for others)