Fig. 3

Transcriptomic analysis by RNA-Seq after chronic morphine treatment in mESCs. a Volcano plot of DEGs showing significant genes (p < 0.05 and FDR < 0.05) in purple and not significant in grey. b Gene Ontology analysis showing the top biological functions, performed with the criteria of Bonferroni corrected for p < 0.05. c Numbers of annotated genes form H3K27me3 binding sites (BSs) and differential binding sites (DBSs), and differential expressed genes (DEGs) after 24 h morphine treatment. d Venn diagram showing the overlap between H3K27me3 BSs and DBSs at propotor level, and RNA-seq DEGs after morphine treatment, e Gene Ontology analysis showing the top biological functions, performed with the criteria of Bonferroni corrected for p < 0.05