Fig. 7

Differential effects of apabetalone treatment on IFNγ-sensitive inflammatory mediators and pathways in DM2 + CVD monocytes as compared to controls. a Apabetalone suppresses select IFNγ-induced genes more robustly in monocytes from DM2 + CVD patients as compared to controls. Apabetalone’s effect on gene expression in the presence of IFNγ was calculated relative to IFNγ only condition (100% dotted line). Statistical significance was determined using 2-way repeated measures ANOVA with Bonferroni’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001. b, c Predicted effect of IFNγ and IFNγ + apabetalone treatment on IPA®-curated canonical pathways and upstream regulators. IPA® output was based on the input of gene expression changes of more than 20% with apabetalone treatment (versus DMSO, p < 0.05). IPA® z-scores compare changes in gene expression (“activating” or “inhibiting”) in the experimental dataset to changes predicted by the literature. z < −2 predicts a downregulation and z > 2 predicts an upregulation within a gene set associated with a canonical pathway or a transcriptional regulator. n/d no predicted directional change, IRF interferon response factor; PRRs pattern recognition receptors, RIG-I retinoic acid-inducible gene I