Fig. 4

GCN5 positively regulates HBV replication in the liver. a The levels of HBV DNA, HBeAg, and HBsAg in the supernatant of the medium were measured by qPCR and ELISA in the cells. b The levels of HBV cccDNA were analyzed by selective qPCR 8 dpi in the cells. c HepG2-NTCP and dHepaRG cells were infected with HBV at an MOI of 600 vp/cell and continuously transfected with siGCN5#1 (100 nM) at −4, 0, and 4 dpi. The levels of HBV DNA, HBeAg, and HBsAg in the supernatant of the medium were measured by qPCR and ELISA in the cells. d The levels of HBV cccDNA were analyzed by Southern blot analysis in the cells. e HepG2-NTCP cells and HepaRG cells were infected with HBV at an MOI of 600 vp/cell and continuously transfected with siGCN5#1 (100 nM) at 8 dpi. RT-qPCR analysis was used to quantify the levels of pgRNA. f The mRNA levels of GCN5 were examined by RT-qPCR in the liver from human liver-chimeric mice (n = 3) and HBV-infected human liver-chimeric mice (n = 3). The mean ± SD of at least three experiments is shown. Statistically significant differences are indicated as follows: **P < 0.01; ***P < 0.001