Association between NPPA promoter methylation and hypertension: results from Gusu cohort and replication in an independent sample

Table 3 The associations of NPPA promoter methylation with blood pressure and hypertension in the discovery sample

CpG loci	Systolic blood pressure			Diastolic blood pressure			Prevalent hypertension
CpG loci	β (SE)^a	P	q	β (SE)^a	P	q	OR (95%CI)^b	P	q
Single CpG association
CpG1	− 0.96 (0.29)	< 0.001	0.007	− 0.42 (0.16)	0.009	0.084	0.87 (0.80–0.95)	0.002	0.008
CpG2	0.31 (0.59)	0.604	0.776	0.05 (0.33)	0.882	0.882	0.91 (0.77–1.09)	0.305	0.305
CpG3	− 0.29 (0.39)	0.454	0.681	− 0.24 (0.22)	0.276	0.414	0.88 (0.78–0.98)	0.023	0.042
CpG4	0.01 (0.23)	0.959	0.959	− 0.11 (0.13)	0.379	0.488	0.94 (0.88–1.01)	0.084	0.094
CpG5	0.06 (0.30)	0.850	0.956	− 0.08 (0.17)	0.622	0.700	0.91 (0.83–1.00)	0.042	0.056
CpG6	− 0.48 (0.24)	0.047	0.140	− 0.24 (0.14)	0.076	0.229	0.92 (0.87–0.99)	0.022	0.042
CpG7	− 0.22 (0.23)	0.347	0.625	− 0.18 (0.13)	0.166	0.299	0.93 (0.87–1.00)	0.044	0.056
CpG8	− 0.55 (0.23)	0.018	0.080	− 0.30 (0.13)	0.022	0.098	0.89 (0.83–0.96)	0.001	0.008
CpG9	− 0.21 (0.19)	0.268	0.602	− 0.16 (0.11)	0.137	0.299	0.94 (0.89–0.99)	0.024	0.042
Gene-based association
Average	− 0.43 (0.31)	0.602	-	− 0.29 (0.17)	0.098	-	0.88 (0.81–0.97)	0.008	-
wTPM		< 0.001			0.003			0.002

^aβ indicated the change of blood pressure in mmHg associated with per 5% increase in DNA methylation level, adjusting for age, sex, education level, cigarette smoking, alcohol consumption, body mass index, fasting glucose, low- and high-density lipoprotein cholesterol, and antihypertensive medications
^bOdds of having hypertension associated with per 5% increase in DNA methylation level, adjusting for age, sex, education level, cigarette smoking, alcohol consumption, body mass index, fasting glucose, and low- and high-density lipoprotein cholesterol
The values in bold indicate a statistical significance level at < 0.05

ISSN: 1868-7083