Fig. 4

Tumor-suppressive function of miR-342-3p in SU-DHL-16 cells. a qRT-PCR analysis of miR-342-3p expression at 120 h after transfection. Data were normalized to negative scramble control as 1. b Cellular proliferation upon overexpression of miR-342-3p was analyzed by MTS assay. c Number of dead cells upon overexpression of miR-342-3p was calculated by trypan blue exclusion assay. d Western blotting analysis of LC3A/B protein with SU-DHL-16 cells transfected with miR-342-3p mimics or negative scramble control for 120 h. Numbers above the band represented the relative protein level of LC3A/B-II normalized to β-actin by densitometric analysis with Image J software. e–g SU-DHL-16 cells were exposed to a 24-h treatment with increasing concentrations of 3-methyladenine (3-MA), an inhibitor of autophagy, followed by (e) Western blotting analysis of LC3A/B protein, of which, LC3-II is a functional marker for autophagy, MTS assay (f) and trypan blue exclusion assay (g). Numbers above the band represented the relative protein level of LC3A/B-II normalized to β-actin by densitometric analysis with Image J software. h, i SU-DHL-16 cells were pre-treated with 3-MA (1 mM) for 24 h before transfection with miR-342-3p mimics or negative scramble control for 120 h, followed by MTS assay (h) and trypan blue exclusion assay (i). Data of MTS were normalized to negative scramble control or untreated control as 100%. Data of MTS assay were normalized to negative scramble control as 100%. Columns represent mean +/− 1SD from three experiments in triplicate