Skip to main content

Table 7 Ct values of genes using methylation-specific quantitative real-time PCR

From: Epigenome-wide DNA methylation profiling of preeclamptic placenta according to severe features

Characteristics of DMCs Target gene Method Control PE PE with severe feature P* Bonferroni
Pa Pb Pc
DMCs commonly changed in PE, regardless of severe features HIST1H3E Methyl degree of array 0.201 0.418 0.481 0.001 0.002 0.007 0.590
ΔCt of MSRE-qPCR 4.895 3.638 2.485 < 0.001 0.025 < 0.001 0.056
DMCs specific to severe features of PE TICAM2 Methyl degree of array 0.336 0.511 0.286 < 0.001 < 0.001 0.02 < 0.001
ΔCt of MSRE-qPCR 3.112 2.308 3.231 0.045 0.026 0.736 0.038
ZNF417 Methyl degree of array 0.298 0.452 0.199 < 0.001 < 0.001 0.006 < 0.001
ΔCt of MSRE-qPCR 4.886 3.193 5.811 0.038 0.082 0.312 < 0.001
  1. Data are expressed as means
  2. *Kruskal-Wallis test, followed by the Bonferroni correlation for multiple testing
  3. Pa control versus PE, Pb control versus PE with severe features, Pc PE versus PE with severe features, PE preeclampsia, Ct cycle threshold, MSRE-qPCR real-time quantitative PCR using methylation-specific restriction enzyme