Epigenome-wide DNA methylation profiling of preeclamptic placenta according to severe features

Clinical Epigenetics

Table 7 Ct values of genes using methylation-specific quantitative real-time PCR

Characteristics of DMCs	Target gene	Method	Control	PE	PE with severe feature	P*	Bonferroni
Characteristics of DMCs	Target gene	Method	Control	PE	PE with severe feature	P*	P^a	P^b	P^c
DMCs commonly changed in PE, regardless of severe features	HIST1H3E	Methyl degree of array	0.201	0.418	0.481	0.001	0.002	0.007	0.590
DMCs commonly changed in PE, regardless of severe features	HIST1H3E	ΔCt of MSRE-qPCR	4.895	3.638	2.485	< 0.001	0.025	< 0.001	0.056
DMCs specific to severe features of PE	TICAM2	Methyl degree of array	0.336	0.511	0.286	< 0.001	< 0.001	0.02	< 0.001
	TICAM2	ΔCt of MSRE-qPCR	3.112	2.308	3.231	0.045	0.026	0.736	0.038
	ZNF417	Methyl degree of array	0.298	0.452	0.199	< 0.001	< 0.001	0.006	< 0.001
	ZNF417	ΔCt of MSRE-qPCR	4.886	3.193	5.811	0.038	0.082	0.312	< 0.001

Data are expressed as means
*Kruskal-Wallis test, followed by the Bonferroni correlation for multiple testing
P^a control versus PE, P^b control versus PE with severe features, P^c PE versus PE with severe features, PE preeclampsia, Ct cycle threshold, MSRE-qPCR real-time quantitative PCR using methylation-specific restriction enzyme

ISSN: 1868-7083