Fig. 5

PRDM8 knock out leads to impaired neuronal differentiation. a Representative phase contrast pictures of neuronal differentiations on day 13. Control cells reveal typical aggregates of immature sensory neurons, which are absent in the PRDM8 knockout cells. Scale bar, 200 μM. b Quantitative RT-PCR analysis after 10 days of neuronal differentiation shows upregulation of neuronal markers in control cells, but not in PRDM8 knockouts (color code depicts mean fold change versus GAPDH). c Gene expression changes in RNA-Seq after 27 days of peripheral neuron differentiation (RPM, reads per million; genes with a log2 fold change above 2 or below − 2 are depicted). d CpG sites that are either 20% hypermethylated (red, 1511 CpGs) or hypomethylated (blue, 1738 CpGs) in the PRDM8^−/− clone compared to the isogenic control. e Epigenetic age of control and knockout iPSCs after neuronal differentiation was close to 0 year.