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Table 4 Impact of oocyte vitrification on expression in animals

From: What impact does oocyte vitrification have on epigenetics and gene expression?

References Animal Model Materials Number of oocytes and embryos Technology of assessment Studied sequences Conclusions
Wu et al. [129] Murine MI and MII after IVM from fresh and vitrified GV 20–25 per group RTqPCR Mps1, BubR1, Mad1, Mad2 Expression of spindle assembly checkpoint (SAC)-related genes in GV (Mad1, BubR1, and Mad2), and MII stages (Mps1 and Mad1) were significantly downregulated after vitrification.
Chen et al. [130] Murine fresh and vitrified MII 50 per group RTqPCR Gtl2, Peg10, Sirt1, Peg3, Igf2R, H19, Igf2 Gtl2 and Peg10 were significantly increased, Peg3, Igf2R, and Sirt1 were significantly decreased after vitrification
Jia et al. [127] Porcine MII after IVM from vitrified and fresh COC 25 per group RNAseq
RTqPCR validation
Global analysis (Illumina) 21 genes Significant differential expression between the non-cryopreserved and vitrified oocyte pools (19 upregulated genes and 18 downregulated after vitrification and IVM).
No GO enrichment or KEGG pathway was identified.
Huang et al. [126] Bovine GV, MII after IVM from vitrified GV 3 fresh GV, 4 vitrified GV, 1 fresh MII, and 2 MII derived from vitrified GV RNAseq Global analysis (Illumina) For GV, 12 upregulated genes and 19 downregulated genes after vitrification. No GO enrichment or KEGG pathway was identified.
For MII, 47 upregulated genes and 6 downregulated genes after vitrification. With GO and KEGG analyses, several pathways were identified: transcription regulation, cell differentiation and mitosis, regulation of actin cytoskeleton, and apoptosis.
Ma et al. [131] Bovine MII after IVM from fresh GV, MII after IVM from vitrified GV, fresh GV, vitrified GV 15 per group (*3 experiments) RTqPCR CD9, CD81, DNMT1, and DNMT3b The expression of all analysed genes was downregulated after IVM of vitrified GV when compared to the fresh in vitro matured MII oocytes.
Gao et al. [125] Murine MII after IVM from fresh GV, MII after IVM from vitrified GV, fresh MII, vitrified MII 100 per group RNAseq
RTqPCR validation
Global analysis (Illumina) Atp5e, Atp5o, Ndufb9, Uqcrq, Timm17a,
Dppa5a, H3f3a, Timm13, and Tomm40
No effect of vitrification on the transcriptome. Differences were reported for IVM.
Wu et al. [132] Bovine MII after IVM from vitrified GV (liquid nitrogen-LN or helium-LHe), MII after IVM from fresh GV 120 per group RTqPCR p53, EG5, CDC20, and NPM2 For LN-effet, p53 and EG5 were upregulated after vitrification, and CDC20 was downregulated.
For LHe-effect, lower effect on the expression of some related genes compared to LN vitrification.
Wang et al. [128] Bovine MII after IVM from vitrified GV, MII after IVM from fresh GV 20 per group RNAseq
RTqPCR validation
Global analysis (Illumina) CDK2,UCHL3, CALM, VDAC2, DPH6, MED27, DAD1, MED21, NR1H4, and HMGN1 Significant differential expression between the non-cryopreserved and vitrified oocyte pools (12 upregulated genes and 90 downregulated genes). At GO analysis, several enrichments in terms of membrane–bounded organelles, macromolecular complex, and intra-cellular part were found. No KEGG pathway was identified.
Shirazi et al. [133] Ovine Fresh MII, vitrified MII, fresh GV, vitrified GV 25 per group RTqPCR STAT3, HAT1, HDAC1, SUV39H1, DNMT1, HMGN3a, SMARCAL1, and DNMT3b The HMG3a and HDAC1 expression was downregulated after vitrification.
Zhao et al. [27] Bovine MII after IVM from vitrified GV, MII after IVM from fresh GV 100 per group RTqPCR BAX and BCL2 l1 The BAX expression was upregulated and the BCL2 l1 expression was downregulated after vitrification.
Dai et al. [134] Porcine Fresh MII, vitrified MII 100 per group RTqPCR DNM1, SOD1, MFN2, BAX, and BCL2 The DNM1 expression was upregulated, and the SOD1, MFN2, BAX, and BCL2 were downregulated after vitrification.
Spricigo et al. [135] Bovine MII after IVM from vitrified COC, MII after IVM from fresh COC 20 per group (*4 experiments) RTqPCR DNMT1, SUV39H1, HDAC2, TP53, and CASP3 No effect of vitrification.
Cheng et al. [113] Murine Fresh MII, vitrified MII 100 per group RTqPCR Dnmt1,3a,3b,3 l The DNMTs expression was significantly reduced after vitrification.
Zhao et al. [114] Murine Fresh MII, vitrified MII 200 per group RTqPCR Dnmt1o, Hat1, and Hdac1 promoters No effect of vitrification on the mRNA expression levels of Hat1 and Hdac1.
The Dnmt1o expression was significantly reduced after vitrification.
Zhou et al. [136] Bovine Fresh MII, vitrified MII 50 per group RTqPCR CD9 The CD9 expression was downregulated after vitrification.
Rao et al. [137] Caprine MII after IVM from vitrified and fresh COC 60 per group RTqPCR GDF9, BMP15, TGFBR1, BPR2, BCL2, BAX and P53 No significant differences of most of the genes.
Turathum et al. [138] Canine MII after IVM from vitrified and fresh COC 200 vitrified, 292 fresh RTqPCR HSP70, Dnmt1, SOD1, BAX, and Bcl2 Bcl2 expression was increased after vitrification, whereas BAX was not expressed in both groups.
Anchamparuthy et al. [139] Bovine Fresh GV, MII after IVM from vitrified GV, MII after IVM from fresh GV 25 per group RT-qPCR 18S rRNA, Fas, FasL, Bax, and Bcl-2 The Bax expression was upregulated after vitrification with the ratio of Bax:Bcl-2 elevated.
Habibi et al. [140] Murine MII after IVM from vitrified GV, MII after IVM from fresh GV 10 per group (*3 experiments) RT-qPCR Mater, Sod1, and Hook1 The Mater and Hook1 expression was downregulated after vitrification.
Succu et al. [141] Ovine MII after IVM from vitrified GV, MII after IVM from fresh GV 40 vitrified, 24 fresh RT-qPCR b-actin, H2A.Z histone, Poli A, PAP, HSP90b, P34cdc2, Cyclin B, Na/K-ATPase and Type I cadherin Except for the b-actin and H2A.Z expression, all gene expression was downregulated after vitrification.
  1. GV oocyte at germinal vesicle stage, IVM in vitro maturation, KEGG Kyoto Encyclopedia of Genes and Genomes, LN liquid nitrogen; LHe = liquid helium, MII oocyte at metaphase II stage, GO gene ontology, RNAseq RNA sequencing, RT-qPCR quantitative reverse transcription PCR