Table 1 Impact of oocyte vitrification on DNA methylation in humans
From: What impact does oocyte vitrification have on epigenetics and gene expression?
References | Materials | Number of oocytes and embryos | Technology of assessment | Studied sequences | Conclusions |
|---|---|---|---|---|---|
Liu et al. [109] | Vitrified MII, MII from IVM, GV | 56 in vivo MII, 106 MII after MIV, 122 MII from vitrified GV | Immunofluorescence (5mC) | Global analysis | No significant differences in fluorescence intensities between the three oocyte groups |
De Munck et al. [110] | MII from donated oocytes, sibling cohort | 31 embryos (D3) from 17 fresh MII and 14 vitrified MII | Immunofluorescence (5mC, 5hmC) | Global analysis | No significant differences in fluorescence intensities between embryos from fresh and vitrified oocytes (5mC 1.0 ± 0.49 vs 0.83 ± 0.41; 5hmC 1.0 ± 0.40 vs 0.81 ± 0.36) |
Al-Khtib et al. [111] | MII from IVM (GV donated for research) | 77 MII after IVM from 184 vitrified VG, and 85 MII from 120 fresh GV | Pyrosequencing | H19 (pool of 3 oocytes) and KCNQ1OT1 | Oocyte vitrification at the GV stage does not affect the methylation profiles of H19-DMR et KvDMR1 of the in vitro matured oocytes |