Skip to main content

Table 1 Impact of oocyte vitrification on DNA methylation in humans

From: What impact does oocyte vitrification have on epigenetics and gene expression?

References Materials Number of oocytes and embryos Technology of assessment Studied sequences Conclusions
Liu et al. [109] Vitrified MII, MII from IVM, GV 56 in vivo MII, 106 MII after MIV, 122 MII from vitrified GV Immunofluorescence (5mC) Global analysis No significant differences in fluorescence intensities between the three oocyte groups
De Munck et al. [110] MII from donated oocytes, sibling cohort 31 embryos (D3) from 17 fresh MII and 14 vitrified MII Immunofluorescence (5mC, 5hmC) Global analysis No significant differences in fluorescence intensities between embryos from fresh and vitrified oocytes (5mC 1.0 ± 0.49 vs 0.83 ± 0.41; 5hmC 1.0 ± 0.40 vs 0.81 ± 0.36)
Al-Khtib et al. [111] MII from IVM (GV donated for research) 77 MII after IVM from 184 vitrified VG, and 85 MII from 120 fresh GV Pyrosequencing H19 (pool of 3 oocytes) and KCNQ1OT1 Oocyte vitrification at the GV stage does not affect the methylation profiles of H19-DMR et KvDMR1 of the in vitro matured oocytes
  1. D day of embryo culture, GV oocyte at germinal vesicle stage, 5hmC 5-hydroxymethylCytosine, IVM in vitro maturation, 5mC 5-methylCytosine, MII oocyte at metaphase II stage