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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Circulating unmethylated CHTOP and INS DNA fragments provide evidence of possible islet cell death in youth with obesity and diabetes

Fig. 2

Validation of differentially methylated genes using DNA sequencing. DNA was isolated from Newport Green flow-sorted human β cells (from N = 3 independent donors), EndoC-βH1 human fetal β cell-derived line (from N = 3 independent cultures), and 11 human control tissues (each from a single donor). DNA sequencing was performed after bisulfite conversion on the top 5 differentially methylated genes identified by the Infinium HumanMethylation 450 array. a Heatmap of the relative abundance of methylated (red) and hypomethylated (blue) CpG sites indicated for intergenic, SPATS2, and PPP2R3A genomic locations. b Heatmap of the relative abundance of methylated (red) and hypomethylated (blue) CpG sites indicated for the CHTOP gene

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Clinical Epigenetics

ISSN: 1868-7083

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