Fig. 3

Tet2 deficiency exacerbates renal IR injury in mice. Mice were subjected to sham operation or 60 min of warm kidney ischemia. Blood and kidney tissues were collected at 24 h after reperfusion for the following analyses. S, sham-operated; IR, ischemia-reperfusion injury; WT, wild type; Tet2^−/−, Tet2 knockout mice. a, b Serum creatinine (a) and blood urea nitrogen (b) concentrations of WT and KO mice. *P < 0.05, **P < 0.01, and ***P < 0.001. N = 5 in each group. c, d Representative micrographs showing the morphology of the IR-insulted kidneys from WT (c) and Tet2^−/− (d) mice. Arrows show the injury. e Quantitative assessment of tubular damage. *P < 0.05. N = 5 in each group. f, g The mRNA levels of Kim1 and Ngal were measured by qRT-PCR. *P < 0.05, **P < 0.01, and ***P < 0.001. N = 3 in each group