Fig. 2

Tet2 deficiency impairs the expression of genes associated with cell junctions in the kidney. The kidneys from WT and Tet2^−/− mice (n = 3 in each genotype) were subjected to RNA-seq analysis. a Scatter plotting of the differentially expressed genes in the kidneys between WT and Tet2^−/− mice. Green dot, downregulated gene; red dot, upregulated gene. b Top 10 terms of the pathway analysis of the downregulated genes. c, d Gene set enrichment analyses (GSEA) of RNA-seq data for WT and Tet2^−/− mouse kidneys, revealing the association of the gene program in Tet2^−/− mouse kidneys with the “tight junction” (c) and “adherens junction” (d) gene signature. e qRT-PCR analysis of two representative genes (Tjp1 and Cldn8) in the kidneys from WT and Tet2^−/− mice. *P < 0.05. N = 3 in each group