Fig. 4

ZBTB16 expression inhibits the migration and invasion of carcinoma cells through reversing EMT. a Morphological changes of cells transfected with ZBTB16 or empty vector, obtained by phase contrast microscopy. Original magnification, × 400. b Representative image of wound healing assay in vector- and ZBTB16-expressed MB231 and BT549 cells. c Representative image (left) and the histogram statistics (right) of Transwell cell migration assay in vector- and ZBTB16-expressing MB231 and BT549 cells, × 100 magnification (p < 0.001). p value was assessed by t test. d Representative image (left) and the histogram statistics (right) of Transwell cell invasion assay in vector- and ZBTB16-expressing MB231 and BT549 cells, × 100 magnification (p < 0.001). p value was assessed by t test. e Expression of EMT markers in ZBTB16-expressing carcinoma cells, as determined by qRT-PCR. VIM:Vimentin (f) and Western blot, GAPDH was used as a control. **p < 0.01; ***p < 0.001