Fig. 3
From: Transgenerational inheritance of fetal alcohol exposure adverse effects on immune gene interferon-ϒ
Transgenerational changes in Ifn-e methylation in the spleen after alcohol feeding in pregnant female rats. IFN-pleen after alcohol feeding inwere determined by pyrosequencing in the spleen of F2 male (a), F2 female (b), F3 male (c), and F3 female (d) rat offspring from different germ lines as defined in Fig. 2a. Four different CpGs of the IFN- different CpGs o—CpG1 (-322), CpG2 (-313), CpG3 (-305), and CpG4 (-285) were analyzed. Data are mean + SEM (n = 7–8) and were analyzed using two-way ANOVA with the Bonferroni post-hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 between AFM vs. controls (AD, PFM). F statistics and P values of data shown in figures were presented in Supplementary Table 1