Table 2 Clinical performance of HPV 16 methylation and E6 oncoprotein in triaging HPV 16 positive women for the detection of CIN3+
Triage strategies | Referral rate (%, 95% CI) | Sensitivity (%, 95% CI) | Specificity (%, 95% CI) | PPV (%, 95% CI) | NPV (%, 95% CI) | Youden index (%, 95% CI) | AUC (%, 95% CI) |
|---|---|---|---|---|---|---|---|
Methyl panel A | 39.2 (27.0, 52.9) | 85.7 (60.1, 96.0) | 78.4 (62.8, 88.6) | 60.0 (38.7, 78.1) | 93.5 (79.3, 98.2) | 64.1 (52.2, 76.0) | 0.82 (0.69, 0.91) |
Methyl panel B | 19.6 (11.2, 32.5) | 57.1 (32.6, 78.6) | 94.6 (82.3, 98.5) | 80.0 (49.0, 94.3) | 85.4 (71.6, 93.1) | 51.7 (39.3, 64.1) | 0.76 (0.62, 0.87) |
E6 oncoprotein | 25.5 (15.6, 38.9) | 57.1 (32.6, 78.6) | 86.5 (72.0, 94.1) | 61.5 (35.5, 82.3) | 84.2 (69.6, 92.6) | 43.6 (31.3, 55.9) | 0.72 (0.58, 0.84) |
Methyl panel A+E6 oncoprotein | 45.1 (32.3, 58.6) | 92.9 (68.5, 98.7) | 73.0 (57.0, 84.6) | 56.5 (36.8, 74.4) | 96.4 (82.3, 99.4) | 65.9 (54.1, 77.7) | 0.83 (0.70, 0.92) |
Methyl panel B+E6 oncoprotein | 35.3 (23.6, 49.0) | 78.6 (52.4, 92.4) | 81.1 (65.8, 90.5) | 61.1 (38.6, 79.7) | 90.9 (76.4, 96.9) | 59.7 (47.5, 71.9) | 0.80 (0.66, 0.90) |
Cytology | 66.7 (53.0, 78.0) | 92.9 (68.5, 98.7) | 43.2 (28.7, 59.1) | 38.2 (23.9, 55.0) | 94.1 (73.0, 99.0) | 36.1 (24.1, 48.1) | 0.68 (0.54, 0.80) |
Viral load | 35.3 (23.6, 49.0) | 57.1 (32.6, 78.6) | 73.0 (57.0, 84.6) | 44.4 (24.6, 66.3) | 81.8 (65.1, 91.4) | 30.1 (18.7, 41.5) | 0.65 (0.50, 0.78) |
VIA | 33.3 (22.0, 47.0) | 35.7 (16.4, 61.2) | 67.6 (51.5, 80.4) | 29.4 (13.3, 53.1) | 73.5 (56.9, 85.4) | 3.3 (− 1.1, 7.7) | 0.52 (0.37, 0.66) |