Fig. 1

Radio-resistant cells display alterations of various physiological processes. a Schematic representation of generation of MCF7-RR. b Graph depicting surviving fraction of P and RR at different radiation doses. c Graphical representation of proliferation rate of P and RR. d Real-time PCR based expression analysis of DNA repair–related genes in P and RR. Levels normalized to MCF7-parental. Fold change 1 depicts levels of parental MCF7. e Graph depicting percentage of AnnexinV and Propidium Iodide (PI) dual positive cells in P and RR. f Quantitative representation of cell migration potential of P and RR, plotted as distance migrated after 20 h. g Real-time PCR–based expression analysis of stemness-related genes in P and RR. Levels normalized to MCF7-parental. Fold change 1 depicts levels of parental MCF7. Parental MCF7 is denoted as “P” and radioresistant cell line is denoted as “RR”. Statistical analysis is done by Student’s t test. N = 3 for all experiments. ^*p < 0.05, ^**p < 0.01, n.s.—not significant, a.u.—arbitrary units, Gy—Gray and Hrs.—Hours. Error bars represent ± S.D. of 3 experiments