Fig. 3

Influence of microRNA, H3K27me3, enhancers, LADs, and basal methylation levels, in addition to genomic factors on hypermethylation. a Univariate analysis. The influence of each factor on hypermethylation was estimated by the fraction of hypermethylated genomic blocks. b Influence of basal methylation levels on hypermethylation. The fraction of hypermethylated genomic blocks among the blocks with the same basal methylation levels in HP(−) young gastric mucosa was plotted. Basal methylation levels larger than 0.1 of β value were essential for hypermethylation by aging. In contrast, hypermethylation by inflammation was induced even in genomic blocks with basal methylation levels smaller than 0.1 and was very frequent with basal methylation levels between 0.1 and 0.5. c Multivariate analysis involving locations against a gene and a CGI, microRNA, H3K27me3, enhancers, LADs, and basal expression levels. Boldface; statistically significant, underline; odds ratio > 2.0 or < 0.5