Fig. 2

Heterogeneity of methylation between biopsy samples from patients with associated cancer versus those without. Pyrosequencing was performed on biopsy samples as described. a Mean value of coefficient of variations from 4 samples for each patient in different cohort. The coefficient of variation (CV) was performed to quantify the variability among each of the patients with four samples using a one-way ANOVA, p < 0.05 was considered significantly different between the TA and NTA groups. b-f This decreased clustering is noted when additional biopsies (4+) are compared at discrete loci. One CG with the highest predictive accuracy for each gene was selected, ten patients from each group were presented, and the error bar is shown as mean ± SE