Table 3 ANKRD26 mRNA levels and methylation percentage of the CpG sites, − 689, − 659, and − 651, in MHO (n = 5) and MUO (n = 15) individuals. Obese subjects have been classified as MHO or MUO based on Wildman et al. [32]. In particular, metabolically unhealthy obese subjects are defined by the presence of two or more of these 6 criteria: blood pressure systolic ≥ 130 mmHg and/or diastolic ≥ 85 mmHg or use of anti-hypertensive drugs; fasting triglycerides ≥ 150 mg dl⁻¹ or use of lipid-lowering drugs; fasting HDL-C ≤ 40 mg dL⁻¹ in men and ≤ 50 mg dL⁻¹ in women or use of lipid-lowering drugs; fasting glucose ≥ 100 mg dl⁻¹ or use of anti-diabetic drugs; HOMA-IR > 90^th percentile; and CRP > 90^th percentile, while metabolically healthy obese subjects are defined by the presence of 0 or 1 of the previous criteria. For symmetrically distributed variables, data are shown as mean ± SD and the statistical difference between the two groups was tested by two-tailed unpaired Student’s t test. For skewed distributions, data are shown as median (first quartile—Q1; third quartile—Q3) and statistical differences between the two groups were tested using Mann-Whitney U test. HDL-C, high-density lipoprotein cholesterol; TG, triglyceride; SBP, systolic blood pressure; DBP, diastolic blood pressure; CRP, C-reactive protein; HOMA-IR, homeostatic model assessment of insulin resistance

From: Epigenetic silencing of the ANKRD26 gene correlates to the pro-inflammatory profile and increased cardio-metabolic risk factors in human obesity