Fig. 2

Comparison of S5 methylation classifier in histologically negative (NEG), CIN1, CIN2, CIN3, and cervical cancer cases (CC). The S5 classifier was significantly different between the following group comparisons: NEG vs CIN2 (p = 0.01), NEG vs CIN3 (p < 0.001), NEG vs CC (p < 0.001), CIN1 vs CIN2 (p = 0.03), CIN1 vs CIN3 (p < 0.001), CIN1 vs CC (p = 0.004), CIN2 vs CC (p = 0.007), and CIN3 vs CC (p = 0.02). Other comparisons were not significant (NEG vs CIN1 and CIN2 vs CIN3). Abbreviations: NEG, histologically negative; CIN, cervical intraepithelial neoplasia (of grades 1, 2, and 3); CC, cervical cancer. The top of box represents the upper quartile (p75), bottom the lower quartile (p25), and the line the median (p50). The upper whisker extends to the largest point of the inter-quartile range from the upper quartile. The lower whisker extends to the smallest point of the inter-quartile range from the lower quartile. The outliers are plotted as individual points for each lesion grade. The Cuzick test for trend was highly significant (p < 0.001)