|
Human Methylation450K BeadChip for discovery group
|
MassARRAY EpiTYPER for validation group
|
---|
MCI
|
Controls
|
FDR adjusted P value
|
MCI
|
Controls
|
FDR adjusted P value
|
---|
N = 7
|
N = 5
|
N = 102
|
N = 68
|
---|
General characteristics
|
Age
|
60.0 ± 2.4
|
61.4 ± 2.9
|
0.481
|
59.6 ± 3.0
|
59.6 ± 3.4
|
1.000
|
Gender (M/F)
|
3/4
|
2/3
|
1
|
40/62
|
40/28
|
0.041*
|
Education years
|
9.1 ± 3.3
|
10.8 ± 1.6
|
0.474
|
9 (9, 12)
|
11 (9, 15)
|
0.582
|
BMI (kg/m2)
|
26.0 ± 2.3
|
24.1 ± 2.4
|
0.386
|
24.2 ± 2.5
|
25.0 ± 2.7
|
0.134
|
Current smoker, n(%)
|
3 (42.9%)
|
2 (40.0%)
|
1
|
25 (24.5%)
|
25 (36.8%)
|
0.226
|
Current drinker, n(%)
|
1 (14.3%)
|
2 (40.0%)
|
0.572
|
19 (18.6%)
|
32 (47.1%)
|
< 0.001*
|
Diabetes, n(%)
|
3 (42.9%)
|
0 (0.0%)
|
0.407
|
20 (19.6%)
|
8 (11.8%)
|
0.375
|
Hypertension, n(%)
|
3 (42.9%)
|
2 (40.0%)
|
1
|
31 (31.4%)
|
22 (32.3%)
|
0.842
|
CHD, n(%)
|
2 (28.6%)
|
0 (0.0%)
|
0.416
|
5 (4.9%)
|
2 (2.9%)
|
0.691
|
Cognitive performance
|
MMSE
|
28.0 (24.0, 29.0)
|
28.0 (29.0, 29.0)
|
0.411
|
28.0 (27.0, 29.0)
|
28.5 (27.0, 30.0)
|
0.226
|
MoCA
|
20.3 ± 2.8
|
26.6 ± 0.9
|
0.005*
|
22.4 ± 3.0
|
26.1 ± 2.3
|
< 0.001*
|
AVLT-IR
|
13.3 ± 4.1
|
16.8 ± 2.8
|
0.323
|
15.3 ± 4.5
|
18.4 ± 4.7
|
< 0.001*
|
AVLT-SR
|
4.6 ± 3.6
|
6.4 ± 1.5
|
0.474
|
5.1 ± 2.7
|
6.4 ± 2.3
|
0.003*
|
AVLT-LR
|
3.9 ± 4.0
|
3.8 ± 2.7
|
0.96
|
4 (2, 7)
|
6 (3, 8)
|
0.007*
|
SDMT
|
30.4 ± 14.3
|
41.2 ± 8.6
|
0.336
|
33 (25, 40)
|
37 (31, 46)
|
0.015*
|
LMT
|
8.0 ± 3.6
|
16.2 ± 3.1
|
0.010*
|
10.0 (5.5, 13.0)
|
14.5 (10.0, 16.5)
|
< 0.001*
|
DSF
|
6.5 ± 1.0
|
8.8 ± 0.8
|
0.010*
|
7.2 ± 1.3
|
7.9 ± 1.5
|
0.008*
|
DSB
|
4.3 ± 1.0
|
4.8 ± 0.4
|
0.474
|
4 (3, 5)
|
4 (4, 5)
|
0.016*
|
Dietary B vitamin intakes
|
Vitamin B6 (mg/day)
|
1.9 (1.4, 2.0)
|
2.6 (1.5, 2.8)
|
0.596
|
2.2 (1.8, 2.6)
|
2.2 (1.7, 2.7)
|
0.720
|
Folate (μg/day)
|
311.6 (203.6, 496.4)
|
577.2 (239.4, 705.0)
|
0.596
|
307.5 (242.6, 442.7)
|
376.7 (271.8, 532.5)
|
0.019*
|
Vitamin B12 (μg/day)
|
1.2 (0.3, 2.8)
|
3.2 (1.8, 3.9)
|
0.427
|
2.0 (1.3, 2.9)
|
2.0 (1.2, 3.1)
|
0.936
|
Serum B vitamin biomarkers
|
Vitamin B6 (ng/L)
|
650.3 ± 125.0
|
542.3 ± 108.5
|
0.326
|
618.6 (531.2, 709.2)
|
596.5 (514.0, 645.9)
|
0.193
|
Folate (μg/L)
|
15.0 ± 5.6
|
30.2 ± 3.7
|
0.003*
|
17.4 (11.2, 22.8)
|
32.2 (28.0, 35.6)
|
< 0.001*
|
Vitamin B12 (ng/L)
|
184.0 (113.0, 305.0)
|
284.0 (216.0, 333.5)
|
0.573
|
211.5 (174.8, 262.9)
|
198.5 (143.5, 256.3)
|
0.321
|
Hcy (μmol/L)
|
22.5 ± 2.9
|
9.8 ± 2.8
|
< 0.001*
|
21.1 (18.3, 23.4)
|
9.4 (6.7, 11.9)
|
< 0.001*
|
Oxidative biomarkers
|
ROS (IU/ml)
|
1000.8 ± 345.3
|
779.1 ± 424.8
|
0.474
|
1043.8 (780.2, 1209.5)
|
728.0 (493.8, 964.8)
|
< 0.001*
|
MDA (mmol/L)
|
10.0 ± 1.7
|
7.0 ± 3.5
|
0.225
|
9.7 ± 2.8
|
8.1 ± 2.6
|
0.003*
|
8-OHdG (ng/L)
|
338.1 ± 112.2
|
185.2 ± 46.3
|
0.061
|
271.0 (201.1, 341.1)
|
151.2 (125.9, 176.7)
|
< 0.001*
|
8-iso-PGF2α (ng/L)
|
221.6 ± 59.1
|
118.2 ± 56.22
|
0.049*
|
188.0 (152.0, 279.6)
|
114.0 (90.3, 144.3)
|
< 0.001*
|
- MMSE mini-mental state examination, MoCA Montreal Cognitive Assessment, AVLT-IR auditory verbal learning test-immediate recall, AVLT-SR auditory verbal learning test-short recall, AVLT-LR auditory verbal learning test-long recall, SDMT symbol digit modalities test, LMT logical memory test, DSF digit span forwards, DSB digit span backwards, BMI body mass index, CHD coronary heart disease, Hcy homocysteine, ROS reactive oxygen species, MDA malondialdehyde, 8-OHdG 8-hydroxy-desoxyguanosine, 8-iso-PGF2α 8-iso-prostaglandin F2α, FDR false discovery rate
- Data shown as median (interquartile range) were compared between two groups using the Mann—Whitney U test
- Data shown as mean ± standard deviation were compared between two groups using the Student t test
- Data shown as n (%) were compared between two groups using the chi-square test or Fisher’s exact test
- ∗P < 0.05