Fig. 7

DNA methylation and mRNA expression at the Ddo promoter region. a The Ddo gene structure with the analyzed CpG sites is shown. White box indicates the putative upstream regulatory region; black box indicates the first exon; and gray box indicates the first intron. Black arrows on the top indicate the forward and reverse primers used for bisulfite amplification. The CpG sites position refers to the TSS (+ 1). The Ddo sequence was retrieved by Ensembl with accession number: ENSMUSG00000063428. b The average Ddo methylation is presented for HIPP, CX and CB at each developmental stage. Statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test. Alpha was considered significant at ≤ 0.001. c The average methylation (%) calculated at all seven CpGs is shown for each brain region during ontogenesis. d Ddo mRNA levels are reported for all brain regions and developmental times. Ddo mRNA expression is normalized to the mean values for two housekeeping genes and expressed as 2^–∆Ct values. One-way ANOVA followed by Tukey’s multiple comparison post-hoc test was used to assess statistical significance. *p ≤ 0.001