Fig. 5

SEPT9 interacts with HMGB1 and enhances HMGB1-RB mediated transcription. a, d Nuclear extracts were prepared from HeLa and subjected to IP/Western blotting analyses. Endogenous association of SEPT9 and HMGB1, HMGB1, and RB. Representative results are shown from three independent experiments. b Representative images of immunohistochemical staining of HMGB1 in the cancer tissues that were stained by SEPT9. c The expression of SEPT9 is negatively correlated with HMGB1 expression. e SEPT9 regulates HMGB1 to affect RB, E2F1, p16, cyclin D1, and cyclin A2