Fig. 3

Analysis of EBF3 and TBC1D16 methylation in colorectal cancer. The differentially methylated EBF3 and TBC1D16 CpG sites were analysed in Illumina 450k array (a) and RRBS (b) DNA methylation datasets of colorectal cancer. a Qu et al. Illumina 450k array dataset (accession number GSE77954 [17]) for normal colon (N, n = 11, in blue), adenomas (A, n = 12, in red), carcinomas (C, n = 13, in green), and liver metastases (M, n = 9, in magenta). b The differentially methylated fragment in the EBF3 promoter (chr10:131763530-131763587) identified by Chatterjee et al. [9] and the fragment in TBC1D16 (chr17:77924597–77924683) directly overlapping the cg17295878 site (green) identified by Vizoso et al. [13] were analysed in an RRBS dataset (accession number GSE95654) of normal colon (N, n = 4, in blue), normal colonic crypt (NC, n = 8, in red), aberrant crypt foci (AC, n = 9, in green) and primary colorectal cancer tumour (P, n = 10, in magenta); *P < 0.05, **P < 0.01, ***P < 0.0001