Fig. 7
From: The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells
RNF40 knockdown cells with restored growth capacity show decreased tumorigenic properties. The effect of RNF40 knockdown in combination with Z-VAD treatment was tested in three independent experiments. a The clonogenic potential of HCT116 siRNF40 cells was reduced compared to wild-type controls. This phenotype was not rescued after Z-VAD-mediated caspase inhibition (n = 2). Mean ± SEM, one-way ANOVA. b Migration potential was tested using a trans-well migration assay for 48 h. Z-VAD treatment increased the proportion of migrating cells under RNF40 knockdown conditions which was still significantly lower compared to wild-type cells (n = 3). Mean ± SEM, one-way ANOVA