Fig. 3

Cell morphology and differentially regulated genes suggest apoptotic processes in RNF40 knockdown cells. a A higher proportion of non-adherent cells was observed after the siRNA-mediated knockdown of RNF40. Scale bar, 1 mm. b The same number of cells was seeded prior to the knockdown, and 72 h later, the number of floating and attached cells was counted in three independent experiments. The relative number of non-adherent cells was significantly higher after the loss of RNF40. Mean ± SEM, Student’s t test. c Gene set enrichment analysis indicated increased apoptotic processes after RNF40 knockdown in HCT116 cells