Fig. 2

The loss of ARID1A impairs proliferation of a subset of colorectal cancer cell lines. To mimic ARID1A-deficient colorectal cancer, we used CRISPR/Cas9-mediated gene editing to delete exon 5 of the ARID1A gene from four colorectal cancer cell lines HCT116, DLD1, COLO320DM, and HT29. Regions flanking exon 5 were targeted by two guide RNAs leading to an out of frame gene product. Genotyping PCRs show a shorter product of 761 bp in the ARID1A knockout (KO) cells (a, middle panel). This led to a complete loss of the ARID1A protein from the KO cells (a, lower panel). HSC70 is used as a loading control. Representative graphs show that the proliferation of the HCT116 and DLD1 cell lines is significantly impaired by the deletion of ARID1A, whereas the proliferation of COLO320DM and HT29 is unaffected by this knockout (b). Error bars represent standard deviation between the replicates. n for proliferation assay = 2, *p < 0.05 unpaired t test