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Table 2 Pyrosequencing analysis of individual CpGs in whole cord blood, CD14+ monocytes, and CD235+ nRBCs

From: Smoking-associated AHRR demethylation in cord blood DNA: impact of CD235a+ nucleated red blood cells

Chromosome position Whole cord blood CD14+ monocytes CD235a+ nRBCs
chr5:373378 chr5:373398 chr5:373476 chr5:373490 chr5:373378 chr5:373398 chr5:373476 chr5:373490 chr5:373378 chr5:373398 chr5:373476 chr5:373490
Methylation (%), nonsmoker (n = 19), mean ± SEM 77.5 ± 1.3 78.6 ± 1.4 78.0 ± 1.8 67.5 ± 1.8 77.5 ± 1.6 79.5 ± 1.7 78.9 ± 2.0 65.6 ± 2.0 60.3 ± 1.9 61.1 ± 1.9 60.4 ± 2.1 52.5 ± 2.0
Methylation (%), smoker (n = 34), mean ± SEM 68.1 ± 1.0 69.4 ± 1.0 65.9 ± 1.4 54.6 ± 1.4 65.7 ± 1.2 68.2 ± 1.3 63.9 ± 1.5 49.0 ± 1.5 52.6 ± 1.4 53.3 ± 1.4 50.4 ± 1.6 39.6 ± 1.5
Delta methylation (%) − 9.4 − 9.2 − 12.2 − 12.9 − 11.8 − 11.3 − 15.0 − 16.7 − 7.7 − 7.8 − 10.0 − 12.9
p value (two-sided t test) 7.0E−09* 7.8E−08 5.5E−08 1.8E−07 5.7E−09 7.8E−08 2.2E−09 7.6E−10 4.8E−04 4.8E,04 8.5E−05 3.2E−07
  1. *The statistical significance cutoff following adjustment for multiple comparisons is p < 0.0125