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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Epigenetic silencing of miR-340-5p in multiple myeloma: mechanisms and prognostic impact

Fig. 1

Methylation of RNF130/miR-340 in healthy controls and HMCLs. a Direct sequencing of M-MSP products from positive control with methylated DNA showed the conversion of all unmethylated cytosines into uracils (turned into thymidines after PCR) but all methylated cytosines remained unchanged, indicating the completeness of bisulfite conversion and specificity of MSP. b M-MSP and U-MSP showed all healthy controls (N1-N12) were completely unmethylated (UU), whereas positive control with methylated DNA was completely methylated (MM). c M-MSP and U-MSP showed RNF130/miR-340 was MM in HMCLs, including WL-2 and RPMI-8226R; partially methylated (MU) in JJN-3, KMS-12-PE, MOLP-8, OCI-MY5, OPM-2, and RPMI-8226; and UU in LP-1, NCI-H929, U-266, MMKKF, MMLAL, KMS-11/BTZ, and OPM-2/BTZ

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