Fig. 4

UHRF1 depletion plus HDAC inhibition restore the expression of epigenetically silenced genes in CRC cells. a RT-PCR analysis of UHRF1 and epigenetically silenced genes in DLD1 (left) and RKO cells (right). Cells were transfected with the indicated siRNAs and incubated for 48 h (DLD1) or 72 h (RKO), after which the cells were treated with or without TSA for 24 h. Cells treated with 5-aza-dC are shown as positive controls for gene reactivation. b qRT-PCR analysis of epigenetically silenced genes in the same samples used in a. The results are normalized to GAPDH expression. Shown are means of three replications; error bars represent SDs