Fig. 1

UHRF1 depletion induces global DNA demethylation in CRC cells. a Summaries of UHRF1 expression in normal colon and primary CRC tumors in TCGA datasets (RSEM-normalized count). ***P < 0.001. b Summaries of UHRF1 expression in CIMP-high (CIMP-H), CIMP-low (CIMP-L), and CIMP-negative (CIMP-N) CRCs in TCGA datasets. **P < 0.01, ***P < 0.001. c qRT-PCR analysis of UHRF1 in CRC cell lines and normal colonic tissue. Results are normalized to ACTB expression. Shown are means of three replications; error bars represent SDs. d qRT-PCR showing UHRF1 knockdown in CRC cells. Cells were transfected with control siRNA (siCONT) or siRNAs targeting UHRF1 and were harvested 72 h (DLD1) or 96 h (RKO) after transfection. Results are normalized to GAPDH expression. Shown are means of three replications; error bars represent SDs. ***P < 0.001. e Western blot analysis showing UHRF1 knockdown in CRC cells. The results were confirmed in two independent experiments, and representative results are shown. f Dot blot analysis of 5-methylcytosine (5-mC) in CRC cells transfected with the indicated siRNAs. The results using a control IgG are shown as loading controls. The results were confirmed in two independent experiments, and representative results are shown. g Bisulfite pyrosequencing of repetitive elements in CRC cells transfected with the indicated siRNAs