Fig. 3

TREX2 protein expression in laryngeal cancer is associated with DNA methylation of the TREX2 DMR. a–c Representative images of IHC staining for TREX2 in laryngeal tumor and adjacent non-tumor tissues with different degrees of TREX2 DNA methylation. Images show two laryngeal tumor tissues with reduced TREX2 methylation (a, b) and one adjacent normal tissue sample with high methylation (c). H-scores ranging from 0 (nuclear TREX2 very low) to 3 (nuclear TREX2 very high) are indicated. d TREX2 H-score in tumor (T) and adjacent normal (A) tissue samples. H-score is plotted as continuous variable based on evaluation of TREX2 staining in cell nuclei. e TREX2 H-scores and corresponding TREX2 DMR methylation (amplicon TREX2_2, see Fig. 1b) in laryngeal tumor (T) and adjacent non-tumor tissue (A) samples. Box-whisker plots show mean and 10 to 90 percentile