ZBTB12 DNA methylation is associated with coagulation- and inflammation-related blood cell parameters: findings from the Moli-family cohort

Table 4 Association between ZBTB12 CpG-specific methylation and blood cell functional parameters

Factor n.	CpG n.	Coagulation time			TNFɑ-stim. coagulation time			Delta coag. time (basal-TNF)			Platelet–monocyte aggregates			Platelet–PMN aggregates
Factor n.	CpG n.	Beta	SE	p	Beta	SE	p	Beta	SE	p	Beta	SE	p	Beta	SE	p
F1	3–4	0.018	0.045	0.690	− 0.019	0.050	0.711	− 0.040	0.049	0.411	0.074	0.039	0.061	0.006	0.043	0.898
	26	− 0.007	0.044	0.877	− 0.052	0.049	0.286	− 0.062	0.048	0.201	0.097	0.039	0.013	− 0.003	0.043	0.942
	1	0.023	0.045	0.608	0.038	0.049	0.447	− 0.002	0.048	0.974	0.055	0.039	0.164	− 0.023	0.043	0.602
	11	0.109	0.046	0.018	0.074	0.051	0.148	− 0.063	0.051	0.218	0.016	0.041	0.703	− 0.016	0.046	0.735
	5	− 0.031	0.046	0.501	0.013	0.050	0.798	0.066	0.049	0.179	0.068	0.042	0.102	0.067	0.046	0.142
	27	− 0.040	0.047	0.402	− 0.017	0.052	0.743	0.020	0.052	0.704	0.087	0.042	0.040	0.041	0.046	0.366
	18–20	0.033	0.044	0.461	0.045	0.048	0.353	− 0.005	0.048	0.912	0.037	0.039	0.342	0.030	0.044	0.496
	6	− 0.043	0.044	0.331	− 0.063	0.048	0.191	− 0.004	0.047	0.932	0.026	0.039	0.502	− 0.052	0.043	0.227
F2	9–10	0.104	0.044	0.018	0.154	0.048	0.002*	0.052	0.048	0.274	0.028	0.039	0.478	0.003	0.043	0.951
	21	0.030	0.046	0.505	0.045	0.050	0.368	0.014	0.049	0.771	0.036	0.040	0.369	− 0.008	0.044	0.863
	16	0.052	0.043	0.224	0.134	0.047	0.005*	0.106	0.046	0.023	− 0.012	0.038	0.757	− 0.010	0.043	0.820
	8	0.075	0.044	0.091	0.126	0.048	0.010*	0.061	0.047	0.197	0.031	0.040	0.438	0.019	0.044	0.669
	7^§	− 0.008	0.046	0.859	− 0.069	0.050	0.169	− 0.086	0.046	0.062	− 0.050	0.041	0.230	− 0.039	0.044	0.380

Model adjusted by age, gender, and smoking as fixed effects and family stratification as a random effect; additional covariates were added to the model and were associated to both methylation factors and phenotypes (for blood cell counts, alcohol, and obesity). Standardized values of phenotypes and methylation are reported (beta values reported as standard deviation units). Significant p values are shown in italics
*pFDR significant
^§Factor loading for both Factor 1 and 2 lower than 0.40

ISSN: 1868-7083