Fig. 4

TLR4 is a target gene of RFX1. a Relative luciferase activities in HEK293T cells cotransfected with RFX1 or with empty vector (negative control) and TLR4-luciferase reporter vectors. b Gel electrophoresis of ChIP-PCR product indicated the binding of RFX1 to the TLR4 promoter in CD14⁺ monocyte cells. c ChIP-qPCR indicated the binding of RFX1 to the TLR4 promoter in CD14⁺ monocytes. All values are the average of at least three biological replicates, and the data shown are the means ± SDs. *P < 0.05; **P < 0.01 relative to control