Fig. 1

RFX1 and TLR4 expression changes in CD14⁺ monocytes from CAD patients and in the LDL-treated CD14⁺ monocytes. a The RFX1 mRNA expression level was determined by RT-qPCR in CD14⁺ monocytes from CAD patients (n = 16) and from non-CAD controls (n = 14). b The RFX1 protein expression level was determined by Western blotting in CD14⁺ monocytes from CAD patients (n = 10) and non-CAD controls (n = 10). c The TLR4 mRNA expression level was determined by RT-qPCR in CD14⁺ monocytes from CAD patients (n = 16) and from non-CAD controls (n = 14). d The correlation analysis between RFX1 mRNA levels and TLR4 mRNA levels. e The fold change of RFX1 mRNA expression was determined by RT-qPCR in the LDL-treated CD14⁺ monocytes compared with negative controls (/Control). f The fold change of RFX1 protein expression was determined by Western blotting in the LDL-treated CD14⁺ monocytes compared with negative controls (/Control). g The fold change of TLR4 mRNA expression was determined by RT-qPCR in the LDL-treated CD14⁺ monocytes compared with negative controls (/control). The values are the average of at least three biological replicates in e, f, and all data shown are the means ± SDs. *P < 0.05; **P < 0.01 relative to control