Table 2 Primer list for PCR of BSgDNA
| Locus | 1st PCR primer pair (5′-3′) | 2nd PCR primer air (5′-3′) |
|---|---|---|
| IGF2-H19 ICR |
F–AGGTGTTTTAGTTTTATGGATGATGG [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47] |
F–TGTATAGTATATGGGTATTTTTGGAGGTTT [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47] |
| DLK1-MEG3 IG-DMR (CG6) |
F–TGGGAATTGGGGTATTGTTTATAT R–AAACAATTTAACAACAACTTTCCTC |
F–GTTAAGAGTTTGTGGATTTGTGAGAAATG [39] R–CTAAAAATCACCAAAACCCATAAAATCAC [39] |
| MEG3 DMR (CG7) |
F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CTCATTTCTCTAAAAATAATTAACC | F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CCCCAAATTCTATAACAAATTACT [39] |
| MEG3 promotor (CG9) |
F–TGAGGAAGTAGGGGTTTATAGAGAG R–AACCCTACAACCCCACAAAA [39] |
F–GGAGAGTGGGGTTTATTGTGAA [39] R–AACCCTACAACCCCACAAAA [39] |
| MEG3 intragenic (CG8) |
F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT |
F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT |
| ZAC DMR |
F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48] |
F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48] |
| PEG1 DMR |
F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–AAAAATAACACCCCCTCCTCAAAT [47] |
F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–CCCAAAAACAACCCCAACTC [47] |
| PEG3 DMR |
F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49] |
F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49] |