Table 2 Primer list for PCR of BSgDNA
Locus | 1st PCR primer pair (5′-3′) | 2nd PCR primer air (5′-3′) |
|---|---|---|
IGF2-H19 ICR | F–AGGTGTTTTAGTTTTATGGATGATGG [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47] | F–TGTATAGTATATGGGTATTTTTGGAGGTTT [47] R–TCCCATAAATATCCTATTCCCAAATAACC [47] |
DLK1-MEG3 IG-DMR (CG6) | F–TGGGAATTGGGGTATTGTTTATAT R–AAACAATTTAACAACAACTTTCCTC | F–GTTAAGAGTTTGTGGATTTGTGAGAAATG [39] R–CTAAAAATCACCAAAACCCATAAAATCAC [39] |
MEG3 DMR (CG7) | F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CTCATTTCTCTAAAAATAATTAACC | F–TTATTTTTTTGAATAATAAGAGAAAGTATG R–CCCCAAATTCTATAACAAATTACT [39] |
MEG3 promotor (CG9) | F–TGAGGAAGTAGGGGTTTATAGAGAG R–AACCCTACAACCCCACAAAA [39] | F–GGAGAGTGGGGTTTATTGTGAA [39] R–AACCCTACAACCCCACAAAA [39] |
MEG3 intragenic (CG8) | F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT | F–GTTTGAGATTTGTTGGGTATTT [39] R–AATTTAACTAACAAATCACAAATATTAACT |
ZAC DMR | F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48] | F–GGGGTAGTYGTGTTTATAGTTTAGTA [48] R–CRAACACCCAAACACCTACCCTA [48] |
PEG1 DMR | F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–AAAAATAACACCCCCTCCTCAAAT [47] | F–TTGTTGGTTAGTTTTGTAYGGTT [47] R–CCCAAAAACAACCCCAACTC [47] |
PEG3 DMR | F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49] | F–AAAAGGTATTAATTATTTATAGTTTGGT [49] R–AAAAATATCCACCCTAAACTAATAA [49] |