Table 2 LoD of methylated SDC2 DNA using HCT116 genomic DNA samples
From: Early detection of colorectal cancer based on presence of methylated syndecan-2 (SDC2) in stool DNA
DNA concentration (ng) diluted in 2.0 μg of stool genomic DNA | SDC2 detected (%) | Average CT |
|---|---|---|
20 | 24 out of 24 (100) | 19.01 |
2.0 | 24 out of 24 (100) | 22.43 |
0.2 | 24 out of 24 (100) | 25.55 |
0.1 | 24 out of 24 (100) | 26.92 |
0.05 | 23 out of 24 (95.8) | 28.62 |
0.02 | 22 out of 24 (91.7) | 30.08 |
0.01 | 13 out of 24 (54.2) | 30.39 |
0 | 0 out of 24 (0) | Not detected |