Fig. 2

Widespread alterations to DNA methylation levels in cord blood in response to late gestation maternal folic acid supplementation. a Scatterplot comparing mean methylation levels (β values 1 = 100%; 0 = 0% methylation) at individual probes in placebo and treated groups. The 1000 top-ranking sites between groups are highlighted in red: ρ = correlation value. b Probe methylation density plot comparing the distributions of methylation values per sample group. In the treatment group, there is a decrease in the number of fully methylated sites (β > 0.75). c Split in top 1000 ranking sites losing or gaining methylation overall. Also shown are numbers of sites showing changes greater than 5% or 10%. d Top 5 differentially methylated sites overall, sorted by combined rank, the value being computed as the maximum (i.e., worst) value among the mean quotient log, mean difference in methylation and p value (P). No., number; Chr, chromosome; Position, coordinates in hg19 human genome release; CG probe, identity number of the CpG probe on the EPIC array; % change, difference in mean β value expressed as %; Gene, nearest gene; P, probability (uncorrected); Rank, RnBeads computed ranking value (lowest being best)