Table 1 Summary of immunohistochemistry peptide blocking results
From: DNA methylation and histone post-translational modification stability in post-mortem brain tissue
Peptide | Antibody | ||||||
|---|---|---|---|---|---|---|---|
H3K4me3 (Abcam) | H3K4me3 (Active Motif) | H3K9me2, K9me3 | H3K27me2 | H3K27me3 | H3K36me3 | H3K27ac | |
H3K4me | [+] | [+] | Â | Â | Â | Â | |
H3K4me2 | [++] | [++] | − |  |  | ||
H3K4me3 | +++ | +++ |  | − | |||
H3K9me | Â | [+] | Â | ||||
H3K9me2 | Â | Â | |||||
H3K9me2, H3K9me3 | ++ | Â | |||||
H3K9me3 | [+] | [++] |  | − | − | ||
H3K27me |  | − | − |  | |||
H3K27me2 | [−] | − |  | ||||
H3K27me3 | [+] | [+] | − | +++ | − | ||
H3K36me |  |  |  | − | |||
H3K36me2 | − |  | − | ||||
H3K36me3 |  | − | +++ | ||||
H3K27ac | − |  | +++ | ||||
H4K5ac |  | − | |||||
Summary | Fairly specific | Fairly specific | Fairly specific | Not specific | Specific | Specific | Specific |