Fig. 4

Ectopic expression of HDAC6 rescues cells from apoptosis induced by MPT0G211/doxorubicin combination. a HDAC6 levels were measured 18 h after transfection with an HDAC6-expression plasmid. b Following co-treatment with MPT0G211 and doxorubicin (DOXO) for 24 h, the levels of the apoptotic proteins poly-ADP ribose polymerase (PARP), caspase 3, γ-H2AX, and acetyl-α-tubulin were measured in HDAC6-overexpressing cells. c Cell cycle distribution was measured in HDAC6-transfected HL-60 cells following treatment with a combination of MPT0G211 and DOXO. A quantitative analysis of the proportions of cells in the sub-G1 phase of cell cycle is shown in d. Data are shown as means ± standard errors of the means. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group